Journal: Frontiers in Neuroscience

Article Title: A Guide to Extract Spinal Cord for Translational Stem Cell Biology Research: Comparative Analysis of Adult Human, Porcine, and Rodent Spinal Cord Stem Cells

doi: 10.3389/fnins.2020.00607

Figure Lengend Snippet: Descriptions of the antibodies used for NSPC characterization including antibody specificity, dilution used, and source.

Article Snippet: β-iii tubulin , Cytoskeletal protein abundant in neural precursors , 1:500 , STEMCELL Technologies.

Techniques: Membrane, Marker

Journal: Frontiers in Neuroscience

Article Title: A Guide to Extract Spinal Cord for Translational Stem Cell Biology Research: Comparative Analysis of Adult Human, Porcine, and Rodent Spinal Cord Stem Cells

doi: 10.3389/fnins.2020.00607

Figure Lengend Snippet: Human, porcine, and rodent primary NSPCs grew in EFH proliferate (BrdU + ) and express neural stem cell marker Sox2. Upon differentiation, NSPCs are multipotent and generate β-iii tubulin + neural precursors, GFAP + astrocytes, and O4 + oligodendrocytes. No O4 + staining was observed from porcine NSPCs. Scale bar = 100 μm.

Article Snippet: β-iii tubulin , Cytoskeletal protein abundant in neural precursors , 1:500 , STEMCELL Technologies.

Techniques: Marker, Staining

Journal: International Journal of Environmental Research and Public Health

Article Title: Increased Expression of Autophagy-Related Genes in Alzheimer’s Disease—Type 2 Diabetes Mellitus Comorbidity Models in Cells

doi: 10.3390/ijerph20054540

Figure Lengend Snippet: Sqstm1 and β-Tubulin III immunoreactivity in hippocampal and cortical sections of wild-type or 3xTg-AD mice at 12 months; scale bar: 20 µm ( A ). Sample Sqstm1 immunoblot ( B ). Sqstm1 levels in 3xTg-AD (3xTg) or wild-type (wt) mice at 6, 12, and 18 months (mo) of age ( C ). The means + SEM are plotted; n = 6/group.

Article Snippet: In immunofluorescence experiments, the following antibodies and dilutions were used: anti-Phospho SQSTM1/p62 (S349) (Abcam, Cambridge, UK, cat # ab211324) 1:100; anti-SQSTM1/p62 (Abcam, cat # ab56416) 1:50; anti-β-Tubulin III (Merck Millipore, Burlington, MA, USA cat # T2200) 1:500; anti-MAP2 (1:500, Merck Millipore, cat # M9942) 1:500; anti-GFAP (Thermo Fisher Scientific, Waltham, MA, USA, cat # 13-0300), 1:800, donkey anti-rabbit-IgG Alexa Fluor 488 (Thermo Fisher Scientific, cat # R37118) 1:1000; donkey anti-mouse-IgG Alexa Fluor 594 (Thermo Fisher Scientific, cat # A-21203, 1:1000); goat anti-mouse IgG1 CF 568 (Merck, cat # SAB4600313 1:1000); goat anti-rat Alexa Fluor 647 (Thermo Fisher Scientific, cat # A21247, 1:1000); and DAPI (4′,6-diamidino-2-phenylindole Merck Millipore, cat #D9542) 1:5000.

Techniques: Western Blot

Journal: Molecular Oncology

Article Title: Downregulation of miR‐326 and its host gene β‐arrestin1 induces pro‐survival activity of E2F1 and promotes medulloblastoma growth

doi: 10.1002/1878-0261.12800

Figure Lengend Snippet: Biological effects of ectopic expression of miR‐326 and ARRB1 in MB CSCs. (A–C) MB CSCs were assayed 48 h after transfection with miR‐326 and ARRB1 , individually or combined. Mock‐transfected cells served as controls. Ectopic expression of ARRB1 and/or miR‐326 in these cells (A) reduced proliferation (MTT assay) (24 h: vs. CTRL: miR‐326+ P = 0.0072; ARRB1‐HA+ P = 0.0245; miR‐326+ARRB1‐HA+ P = 0.0015; 48 h: vs. CTRL: miR‐326+ P = 0.001; ARRB1‐HA+ P = 0.0005; miR‐326+ARRB1‐HA+ P < 0.0001), (B) diminished the frequency of oncosphere‐forming cells (vs. miR‐326‐ARRB1‐HA−: miR‐326+ P = 0.001; ARRB1‐HA+ P = 0.0005; miR‐326+ARRB1‐HA+ P = 0.0003), (C) decreased NANOG expression and increased the expression of PARP‐C (miR‐326 levels: miR‐326+ P < 0.0001; miR‐326+ARRB1‐HA+ P = 0.0002 vs. miR‐326‐ARRB1‐HA−). (D) qRT‐PCR revealed significantly increased expression of neuronal and glial differentiation markers ( βIII tubulin and GFAP , respectively) only in MB CSCs overexpressing miR‐326 (alone or with ARRB1 ) (vs. Mock βIII tubulin: miR‐326 P = 0.0027; miR‐326 and ARRB1 P = 0.046, GFAP: miR‐326 P = 0.0002; miR‐326 and ARRB1 P = 0.011). Data represent means ± SD from five independent experiments. Statistics: One‐way ANOVA and two‐way ANOVA, * P < 0.05; **P < 0.01; *** P < 0.001; **** P < 0.0001 vs. indicated controls.

Article Snippet: Cell was stained with mouse monoclonal antibodies against βIII tubulin (MAB 1637; Merck, Darmstadt, Germany) and GFAP (MAB360).

Techniques: Expressing, Transfection, MTT Assay, Quantitative RT-PCR

Journal: iScience

Article Title: KAT6B overexpression in mice causes aggression, anxiety, and epilepsy

doi: 10.1016/j.isci.2025.111953

Figure Lengend Snippet:

Article Snippet: βIII tubulin, clone 5G8 , Promega , G7121.

Techniques: Plasmid Preparation, Isolation, Staining, Control, CRISPR, Recombinant, Software, Fluorescence